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The use of endemic aquatic organisms as biological sentinels has proved useful to environmental monitoring. Eighteen H. After two months the H.

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Key words: Comet assay, micronucleus, chromosome aberrations, tributyltin, lead, fish. The aquatic environment is the ultimate destination for almost all urban, industrial and agricultural wastes. Organic tin compounds such as tributyltin TBT are used for many purposes, principally in antifouling paints and the plastics industry Alzieu, , several reports strongly suggesting that TBT can adversely affect the physiology of not only mammals Mizuhashi et al. In spite of the fact that tin can bind to DNA no direct damage has yet been demonstrated, although some studies have shown apoptosis in mouse timocytes and hepatocytes exposed to different concentrations of TBT Yamanoshita et al.

Lead has been in use for thousand years, although the toxic effects of lead compounds were only recognized less than hundred years ago. Inorganic and organic lead enters the environment in several ways Pain, ; Godwin, and can have diverse effects on organisms, including mimicking essential elements e.

This paper reports the results a study of the neotropical fish Hoplias malabaricus in which this fish was exposed to TBT and PbII and the effects of the exposure assessed using the comet assay and the piscine micronucleus and chromosome aberration tests. This fish has the advantage that it is easily fed under laboratory conditions and, because it devours its pray whole, can be easily exposed to xenobiotics by feeding with a prey species previously injected with the material under investigation, a further advantage being that this species has 40 relatively large bi-armed chromosomes which makes studying the genetic effects of metals easier.

In our study, we used 18 adult H. We exposed our H. The feeding schedule consisted of offering each H. Four days after the last feed-cycle the H.

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For the comet assay, also known as the single-cell gel test SCGE , we used the procedure of Singh et al. Clean microscope slides were prepared by covering them with a thin layer of 0. For each slide, 10 m L of whole fish blood was diluted in 1 mL of bovine calf serum and 10 m L of this mixture placed in m L of LMP agarose gel Gibco which was then spread on the base layer of the slide and a cover slip added, the agarose gel being allowed to solidify by placing the slide in a refrigerator for 10 min.

Comets were scored using a Zeiss epifluorescence microscope. For each fish, cells were visually analyzed according to the method of Kobayashi et al. Forgot password? Old Password.

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